The Center continues to actively carry out substantive collaboration with Chair Professor Robert Turesky at the Masonic Cancer Research Center, University of Minnesota, USA in our development of the DNA adductomics analytical platform. Recently, Dr. Yu-Ming Hsu, as a postdoctoral researcher and member of our Center’s platform team, is performing a one-year research project involving the platform in the laboratory of Dr. Robert Turesky. We expect to gradually implement the specialized analysis platform for DNA adduct identification in our center to explore the interaction and mechanism underlying environmental exposure, genes, and disease in depth. A novel nano-LC-Orbitrap-MS method for DNA adductomic analysis was developed with MS parameters optimized for maximum performance by analyzing 21 aldehyde-related adducts, especially trans,trans-2,4-decadienal (tt-DDE) derived adducts, the most abundant long-chain aldehyde found in Chinese-style COFs. We demonstrated that this DNA adductomics approach could be used successfully in tt-DDE-treated calf thymus DNA (ctDNA) under normal physiological conditions (37oC, pH 7.4) and we discovered that 8 targeted aldehyde-related DNA adducts could be identified simultaneously. In the future, this DNA adductomic method should be found to be a powerful tool for pre-screening purposes to determine targeted mutagenic DNA adducts in in vivo and clinical studies.